PRECISION TECHNOLOGY FOR GLOBAL SECTORS

Summary

We provide a tool primarily for single-cell analysis and for adapted molecular biology needs applied to single cells in a tissue slice or cell culture (the equipment is known as DISCO, Digital microfluidic Isolation of Single Cells for -Omics).

Our long-term goal includes providing predictive -omics capabilities using our system and just images of cells.

Our current business model provides services for using our instrument (service-for-fee), selling the instrument for use by independent research groups (buying a DISCO instrument), and long-term services including subscriptions to our AI-assisted data prediction and user-collected databases.

Our mission:

Bring the researcher back into single-cell -omics

Components of CellOut System

Microscope with single-cell laser lysis capability

Upright fluorescent microscope with port for a Q-switch Nd:YAG laser capable of removing a single cell from a tissue slice or adherent cell culture system

Digital microfluidic capable stage

The microscope stage has integrated digital microfluidic capabilties, enabling collection of single cell lysates in microliter volumes

Custom software

Custom software integrating the laser lysis and imaging modules of the microscope.
With eventual AI-assisted data prediction capabilities

Patents we hold: US 8,367,370 B2, US 8,728,291 B2

OUR SOLUTIONS

DISCO provides a unified platform that links -omics profiles with images that hold their spatial information.

Getting the most from your single cell data

Making single cell -omics more approachable

Users can select cells they want to analyze for downstream -omics with a friendly user interface and the potential for automated collection of cells lysates.

Having a platform for single cell -omics validation

We provide a platform that can perform –omics assays and downstream validation assays (such as immunofluorescence and RNA-FISH)

Publications

Digital microfluidic isolation of single cells for -Omics

This was our first paper showing single cell -mics (genomics, transcriptomics, and proteomics) in in vitro cell culture systems using B16 (murine) and U87 (human)
(Lamanna, J., Scott, E.Y., Edwards, H.S. et al. Digital microfluidic isolation of single cells for -Omics. Nat Commun 11, 5632 (2020). https://doi.org/10.1038/s41467-020-19394-5)

Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice

This was our first paper using DISCO on methanol fixed and stained tissue sections (mouse cortex). We performed single-cell RNA-seq and scProteomics. We also compared our DISCO results to 10X Visium and 10X Chromium data in this paper.
(Scott, E.Y., Safarian, N., Casasbuenas, D.L. et al. Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice. Nat Commun 15, 1584 (2024). https://doi.org/10.1038/s41467-024-45821-y)

The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors

This was our first paper performing DISCO, scRNA-seq on PFA-fixed mouse cortical tissue from different developmental timepoints.
(Lozano Casasbuenas D, Kortebi I, Gora C, Scott EY, Gomes C, Oliveira MS Jr, Sharma T, Daniele E, Olfat A, Gibbs R, Yuzwa SA, Gilbert EA, Küry P, Wheeler AR, Lévesque M, Faiz M. The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors. Glia. 2024 Sep;72(9):1693-1706. doi: 10.1002/glia.24578. Epub 2024 Jun 9. PMID: 38852127.)

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